EmeraldAmp MAX products
The EmeraldAmp Max PCR Master Mix offers a powerful, flexible, and convenient option for high-yield, routine, and specific PCR. This premix formulation includes PCR enzyme, an optimized buffer, dNTPs, gel loading dye (green), and a density reagent in a convenient 2X master mix. Only primer and DNA template need to be added to initiate the reaction. A hot-start version is also available.
EmeraldAmp Max PCR Master Mix can amplify targets up to ~10 kb (genomic DNA) and works well with AT- or GC-rich templates. It yields at least 10 times more end product compared with standard PCR and performs better than products using standard Taq DNA polymerase.
Completed reactions can be analyzed directly via gel electrophoresis. The vivid green dye separates into blue and yellow dye fronts when the PCR product is run on an agarose gel. The dyes can be removed by gel purification or PCR cleanup using the NucleoSpin Gel and PCR Clean-Up kit if necessary. It is recommended that the dye be removed from the gel prior to conducting any downstream fluorescent applications.