pHLys Red - Lysosomal Acidic pH Detection

Description

The lysosome is an organelle in which a biomembrane forms an acid vacuole. Lysosomes contain various degrading enzymes and contribute to maintaining intracellular homeostasis by acting as a waste disposal system. Recent findings reveal that lysosomal dysfunction is related to some neurodegenerative disorders. Consequently, the investigation of lysosomal function is attracting considerable interest in the scientific community.

pHLys Red accumulates in the intact lysosome, and its fluorescence intensity is enhanced as the acidity increases. On the other hand, weak fluorescence is observed when lysosomes are neutralized due to lysosomal dysfunction. Please also consider the Lysosomal Acidic pH Detection Kit (Dojindo code: L266), which includes the lysosome staining dye LysoPrime Green (pH-independent). Lysosomal pH and lysosomal mass can be measured by combining these two dyes, which exhibit pH-dependent (pHLys Red) and pH-independent (LysoPrime Green) behavior in lysosomes.