TB Green Premix Ex Taq (Tli RNaseH Plus), ROX plus

Description

TB Green Premix Ex Taq (Tli RNase H Plus) is an intercalator-based real-time PCR (qPCR) master mix recommended for most qPCR applications, including gene expression analysis, genotyping and high-throughput studies. It is supplied as a 2X premix containing TB Green, so reactions are assembled simply by adding primers, template and sterile distilled water. The premix uses TaKaRa Ex Taq HS, a hot-start PCR enzyme with an anti-Taq antibody, in a buffer optimized for qPCR, giving high amplification efficiency and sensitivity. It also contains Tli RNase H, a heat-resistant RNase H that minimizes qPCR inhibition caused by residual mRNA in cDNA templates. It also contains dNTPs and Mg2+. This version is supplied as a 5 mL bulk format with ROX Reference Dye included (ROX plus).