SPiDER-?Gal

Description

The gene of ?-galactosidase from E. coli is widely used as a reporter gene assay marker. Although X-gal is well known reagent to detect ?-galactosidase in cell or tissue samples, the assay using these reagents require to fix cells or tissues due to the poor cell-permeability. In addition, so far developed the assay using fluorescence reagents cannot clearly differentiate ?-galactosidase-expressed cells or regions. To overcome these issues, Urano, Kamiya and co-workers have successfully developed SPiDER-?Gal. SPiDER-?Gal ideally possesses cell-permeability and the ability to retain in intracellular region.